ORCID
- Brian Gibson: 0000-0002-1270-6532
Abstract
Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market.
DOI Link
Publication Date
2022-01-01
Publication Title
Nature Communications
Volume
13
Issue
1
Deposit Date
2026-07-24
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Additional Links
Recommended Citation
Mozzachiodi, Simone; Krogerus, Kristoffer; Gibson, Brian; Nicolas, Alain; and Liti, Gianni, "Unlocking the functional potential of polyploid yeasts" (2022). Research Outputs: 2025-Present. 70.
https://arrow.tudublin.ie/schfsehro/70