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1.4 CHEMICAL SCIENCES, Analytical chemistry
A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.
Karcz, D. et al (2019) Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors, Int. J. Mol. Sci. 2019, 20(2), 281; DOI: 10.3390/ijms20020281