Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors
Document Type
Article
Rights
Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence
Disciplines
1.4 CHEMICAL SCIENCES, Analytical chemistry
Abstract
A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.
DOI
https://doi.org/10.3390/ijms20020281
Recommended Citation
Karcz, D. et al (2019) Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors, Int. J. Mol. Sci. 2019, 20(2), 281; DOI: 10.3390/ijms20020281
Publication Details
Int. J. Mol. Sci. 2019, 20(2), 281; https://doi.org/10.3390/ijms20020281