Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors

Authors

Karolina Starzak, Cracow University of Technology, Poland
Arkadiusz Matwijczuk, University of Life Sciences in Lublin, Poland
Bernadette Creaven, Technological University DublinFollow
Alicja Matwijczuk, University of Life Sciences in Lublin, Poland
Sławomir Wybraniec, Cracow University of Technology, Poland
Dariusz Karcz, Cracow University of Technology, PolandFollow

Document Type

Article

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

1.4 CHEMICAL SCIENCES, Analytical chemistry

Publication Details

Int. J. Mol. Sci. 2019, 20(2), 281; https://doi.org/10.3390/ijms20020281

Abstract

A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.

DOI

https://doi.org/10.3390/ijms20020281

Recommended Citation

Karcz, D. et al (2019) Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors, Int. J. Mol. Sci. 2019, 20(2), 281; DOI: 10.3390/ijms20020281