Document Type

Article

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

Electrochemistry

Publication Details

IEEE Sensors Journal

Abstract

Here we report an electrochemical ELISA approach for cardiac Troponin T (cTnT) determination based on a lateral flow membrane with underlying screen printed electrodes (<;100 Ω /cm 2 ). The thick film transducer was modified with the anti-cardiac Troponin T antibody via physisorption and the electrochemical performance of the immunosensor was evaluated using cyclic voltammetry. The capture antibody coated immunoelectrode employed for electrochemical determination of Troponin T antigen used a sandwich assay format with horseradish peroxidase conjugated signaling antibodies held in the track of a lateral flow sensor strip. A simple two step procedure realized signal acquisition within <;20 min (total assay time). The lateral flow electrochemical immunosensor response resulted in a calibration curve with linear response (0-700 ng/ml cTnT) with limit of detection of 0.15 ng/ml.

DOI

https://doi.org/10.1109/JSEN.2018.2789436

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