Synergistic cytotoxicity effect of ultrasound and plasma microbubble in glioblastoma 3D tumor sphere model

Authors

Janith Wanigasekara, Technological University Dublin
Patrick J. Cullen, Technological University Dublin
Brijesh Tiwari, Teagasc Food Research Centre
James F. Curtin, Technological University Dublin

Document Type

Article

Publication Details

https://doi.org/10.1002/ppap.202300185

Abstract

The combined application of ultrasound (US) and plasma microbubble (PMB) is a promising technology against glioblastoma (GBM). Here, we determined the effects of US and PMB (alone and combined) on U-251 MG GBM and A431 epidermoid carcinoma spheroids under different conditions. The synergistic treatment of US- and PMB-induced tumor sphere cell death in a time-, dose-, treatment frequency-, and reactive oxygen species dependent manner, while multiple treatments augmented this effect. Synergistic treatments significantly reduced three-dimensional GBM spheroid regrowth and cell proliferation while inducing cytotoxicity, spheroid shrinkage, and damage to the tumor microenvironment. PMB-induced cytotoxicity throughout the tumor sphere, likely via long-lived reactive oxygen and nitrogen species (H2O2, NO2−, and NO3−) and other reactive species.

DOI

10.1002/ppap.202300185

Recommended Citation

Wanigasekara, J., Cullen, P. J., Tiwari, B., & Curtin, J. F. (2024). Synergistic cytotoxicity effect of ultrasound and plasma microbubble in glioblastoma 3D tumor sphere model. Plasma Processes and Polymers, 21(6), Article 2300185. https://doi.org/10.1002/ppap.202300185

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.