Author ORCID Identifier
0009-0000-0630-2200
Document Type
Dataset
Disciplines
Optics, Analytical chemistry, Biophysics
Abstract
This dataset contains raw spectral data obtained from single-cell Raman microspectroscopy under two nutritional conditions: glucose alone and glucose supplemented with glutamine. LLC-MK2 cells were starved for 2 h, then exposed to nutrients and fixed every 15 min for up to 120 min. At each time point, spectra were recorded from 25 individual cells (cytoplasm regions) and exported as machine-readable files (.csv). MATLAB scripts are provided to implement two-dimensional correlation spectroscopy (2D-COS) for generating synchronous maps, along with utilities for loading spectra, averaging, and reproducing key figures. A set of simulated time-series spectra used to validate 2D-COS under high background interference is also included with code.
DOI
https://doi.org/10.21427/186a-gm75
Recommended Citation
Mirveis, Zohreh; Patil, Nitin; and Byrne, Hugh J., "Dataset of Raman spectra and MATLAB code for: Elucidating time-resolved intracellular metabolic dynamics via label-free Raman microspectroscopy and 2D correlation spectroscopy" (2025). Other Resources. 9.
https://arrow.tudublin.ie/scschphyot/9
Funder
Science Foundation Ireland
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Details
Dataset underlying the manuscript submitted to Analyst (RSC): “Elucidating time-resolved intracellular metabolic dynamics via label-free Raman microspectroscopy and 2D-COS.”