Document Type
Article
Abstract
The potential of Raman microspectroscopy of exfoliated cells has been demonstrated for oral cancer diagnosis. In this study, brush biopsies were collected from the buccal mucosa/tongue of healthy donors (n = 31) and from oral mucosal dysplastic lesions (n = 31 patients). Raman spectra were acquired and subjected to partial least squares-discriminant analysis (PLS-DA). The patient samples could be differentiated from healthy donor samples with 96% sensitivity and 95% specificity. Furthermore, PLS-DA models were developed based on cytopathological and histopathological assessment. Low and high grade dysplasia could be discriminated with 64% sensitivity and 65% specificity based on cytopathological assessment, while 81% sensitivity and 86% specificity could be achieved when histopathological assessment was within six months of the brush biopsy sampling. Therefore, this explorative study has successfully demonstrated that Raman spectroscopy may have a role in monitoring patients with dysplasia and may reduce the need for multiple biopsies.
DOI
10.1016/j.saa.2021.120437
Recommended Citation
Behl, I., Calado, G., Vishwakarma, A., Traynor, D., Flint, S., Galvin, S., Healy, C. M., Pimentel, M. L., Malkin, A., Byrne, H. J., & Lyng, F. M. (2022). Classification of cytological samples from oral potentially malignant lesions through Raman spectroscopy. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 266, Article 120437. https://doi.org/10.1016/j.saa.2021.120437
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Publication Details
https://doi.org/10.1016/j.saa.2021.120437