Document Type
Article
Abstract
Purpose: To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. Materials and methods: Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity enhancer, uncoupler or were either melanin or glutamine supplemented/starved for 4 hours pre-exposure to sunlight. A Q-Sun Solar Simulator (Q-Lab, Homestead, FL, USA) was employed to expose cells to simulated sunlight. Confocal microscopy imaging of A375 cells co-loaded with mitochondria and lysosome-specific fluorescent dyes was used to identify these organelles and predict mitophagic events. Results: SSI induces pronounced changes in mitochondrial dynamics and mitophagy in exposed skin cells compared to control and these effects were modified by both glutamine and melanin. Conclusions: Mitochondrial dynamics and rate of mitophagy in melanoma cells are sensitive to even short bursts of environmentally relevant SSI. Mitochondrial dynamics, and its modulation, may also play a role in mitophagy regulation, cell survival and proliferation post SSI.
DOI
10.3109/09553002.2011.556175
Recommended Citation
Zanchetta, L. M., Garcia, A., Lyng, F., Walsh, J., & Murphy, J. E. (2011). Mitophagy and mitochondrial morphology in human melanoma-derived cells post exposure to simulated sunlight. International Journal of Radiation Biology, 87(5), 506-517. https://doi.org/10.3109/09553002.2011.556175
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Publication Details
https://doi.org/10.3109/09553002.2011.556175