Direct Immunoassays and Their Performance: Theoretical Modelling of the Effects of Antibody Orientation and Associated Kinetics

Authors

Dana Mackey, Technological University DublinFollow
Eilis Kelly, Technological University Dublin
Robert Nooney, Dublin City University
Richard O'Kennedy, Harnad Bin Khalifa University

Document Type

Article

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

Applied mathematics

Publication Details

Integrative Biology, issue 10, 2018

Abstract

The orientation and activity of antibodies immobilized on solid surfaces are of direct relevance to many immunosensing applications. We therefore investigate a mathematical model which estimates the fraction of antibodies which are available for reaction in a randomly adsorbed sample. Numerical simulations are presented which highlight the separate effects of antibody orientation, accessibility and loss of binding ability on the amount of captured antigen. The assay response can then be expressed as a function of total antibody density and used for optimizing the surface coverage strategy under various conditions.

DOI

https://doi.org/10.1039/C8IB00077H

Recommended Citation

Mackey, D., Kelly, E., Nooney, R. & O'Kennedy, R. (2018). Direct immunoassays and their performance – theoretical modelling of the effects of antibody orientation and associated kinetics. Integrative Biology, 2018,10, pp.598-604. doi:10.1039/C8IB00077H