Methods to Assess the Direct Interaction of C. Jejuni With Mucins

Authors

Julie Ann Naughton, Technological University DublinFollow
Marguerite Clyne, University College Dublin
Gina Duggan, University College DublinFollow
Billy Bourke, University College DublinFollow

Document Type

Book Chapter

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

1. NATURAL SCIENCES, Microbiology, Biochemistry and molecular biology

Publication Details

Volume 1512 of the series Methods in Molecular Biology pp 107-115

Abstract

Studies of the interaction of bacteria with mucus secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high throughput manner.

DOI

https://doi.org/10.1007/978-1-4939-6536-6_10

Recommended Citation

Naughton, J., Clyne, M., Duggan, G. & Burke, B. (2017). Methods to assess the direct interaction of C. jejuni with mucins. In Methods in Molecular Biology,vol. 1512, pp. 107-115. doi:10.1007/978-1-4939-6536-6_10

Funder

Science Foundation Ireland