Document Type
Article
Disciplines
1.6 BIOLOGICAL SCIENCES
Abstract
This study characterized the physicochemical, technofunctional, and in vitro antioxidant properties along with the AA profile and score of a sprat protein enzymatic hydrolysate (SPH). Furthermore, the impact of the SPH on the growth, proliferation, and muscle protein synthesis (MPS) in skeletal muscle (C2C12) myotubes was examined. The SPH displayed good solubility and emulsion stabilization properties containing all essential and non-essential AAs. Limited additional hydrolysis was observed following in vitro-simulated gastrointestinal digestion (SGID) of the SPH. The SGID-treated SPH (SPH-SGID) displayed in vitro oxygen radical antioxidant capacity (ORAC) activity (549.42 μmol TE/g sample) and the ability to reduce (68%) reactive oxygen species (ROS) production in C2C12 myotubes. Muscle growth and myotube thickness were analyzed using an xCELLigenceTM platform in C2C12 myotubes treated with 1mg protein equivalent.mL−1 of SPH-SGID for 4 h. Anabolic signaling (phosphorylation of mTOR, rpS6, and 4E-BP1) and MPS (measured by puromycin incorporation) were assessed using immunoblotting. SPH-SGID significantly increased myotube thickness (p < 0.0001) compared to the negative control (cells grown in AA and serum-free medium). MPS was also significantly higher after incubation with SPH-SGID compared with the negative control (p < 0.05).
DOI
https://doi.org/10.3389/fnut.2023.1197274
Recommended Citation
Shekoohi, Niloofar; Silotry Naik, Azza; Amigo Benevent, Miryam; Harnedy-Rothwell, Padraigin A.; Carson, Brian P.; and FitzGerald, Richard J., "Physicochemical, Technofunctional, In Vitro Antioxidant, and in Situ Muscle Protein Synthesis Properties of a Sprat (Sprattus Sprattus) Protein Hydrolysate" (2023). Articles. 532.
https://arrow.tudublin.ie/schfsehart/532
Funder
This research was funded under the National Development Plan, through the Food Institutional Research Measure, administered by the Department of Agriculture, Food, and the Marine, Ireland, under grant issue 2019R617
Creative Commons License
This work is licensed under a Creative Commons Attribution-Share Alike 4.0 International License.
Publication Details
https://www.frontiersin.org/articles/10.3389/fnut.2023.1197274/full
Shekoohi N, Naik AS, Amigo-Benavent M, Harnedy-Rothwell PA, Carson BP and FitzGerald RJ (2023) Physicochemical, technofunctional, in vitro antioxidant, and in situ muscle protein synthesis properties of a sprat (Sprattus sprattus) protein hydrolysate. Frontiers in Nutrition, 2023, Jun 23:10:1197274.
doi: 10.3389/fnut.2023.1197274