Document Type
Article
Rights
Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence
Disciplines
Bioprocessing technologies, Health care sciences and services
Abstract
In the present study, BSG was hydrolysed using cellulolytic enzymes and used as a growth medium supplement for cultivation of the thermophilic bacterium, Bacillus stearothermophilus in the production of α-amylase. A central composite design involving five parameters and four levels viz. starch, peptone, KCl, and MgSO4 along with BSG hydrolysate was used to derive the optimal media composition. The fermentation was conducted using shake flasks for 36 h at a temperature of 50 °C and pH 7.0 at 220 rpm. Optimization trials revealed that maximal amylase production (198.09 U/ml) occurred with a medium composition of starch (0.2% w/v), peptone (0.2% w/v), KCl·4 H2O (0.02% w/v), MgSO4·7 H2O (0.01% w/v) and hydrolysate (0.22% v/v). A 1.3-fold increase in amylase activity was obtained following novel media composition. All the factors considered in the study were found to be significant. The enzyme was purified by three step purification strategy, characterised and tested for anti-biofilm activity.
DOI
https://doi.org/10.1016/j.biteb.2019.01.004
Recommended Citation
Rajeev Ravindran, Gwilym A. Williams, Amit K. Jaiswal, Evaluation of brewer's spent grain hydrolysate as a substrate for production of thermostable α-amylase by Bacillus stearothermophilus, Bioresource Technology Reports, Volume 5, 2019, Pages 141-149, ISSN 2589-014X, DOI: 10.1016/j.biteb.2019.01.004.
Funder
Technological University Dublin
Included in
Biotechnology Commons, Food Biotechnology Commons, Food Chemistry Commons, Other Food Science Commons
Publication Details
Bioresource Technology Reports