Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture

Authors

Denis O'Suillivan, Technological University Dublin
Liam O'Neill, Liam O’Neill TheraDep, Questum, Ballingarrane Science and Technology Park, Clonmel, Co. Tipperary
Paula Bourke, Technological University DublinFollow

Document Type

Article

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

3.3 HEALTH SCIENCES

Publication Details

ACS Omega2020, 5, 39, 25069–25076

Abstract

A cold atmospheric plasma unit was used to deposit a biologic, in this case collagen, onto a surface. A collagen coating was applied to 96-well polystyrene plates at a range of powers to determine the effects of the plasma power on the coating structure and viability. Plasma characterization was carried out using voltage, current, and power measurements. Coating characterization was completed using gravimetric measurement, cell growth, water contact angle, as well as spectroscopic analysis and compared to commercial collagen-coated plates. Cell culture studies were also undertaken. The plasma coating matched the performance of the commercial plate but dramatically reduced production time and cost. This method could allow for automated inline production of collagen-coated plates for cell culture applications.

DOI

https://doi.org/10.1021/acsomega.0c02073

Recommended Citation

O'Suillivan, D., O'Neill,L. & Bourke, P. (2020)Direct Plasma Deposition of Collagen on 96-Well Polystyrene Platesfor Cell Culture, ACS Omega2020, 5, 39, 25069–25076 Publication Date:September 24, 2020 DOI:10.1021/acsomega.0c02073