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This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lyso-somal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie bril-liant (CB) blue assay. Supernatants were also assayed for Adenylate Kinase (AK) release and Interleukin 8 (IL-8) which indicated a loss of cell membrane integrity and an in Xammation response respectively.
Davboren, Maria et al: In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells. Toxicology in Vitrovol. 21, no. 3, pp. 438-448. doi:10.1016/j.tiv.2006.10.007