In Vitro Toxicity Evaluation of Single Walled Carbon Nanotubes on Human A549 Lung Cells

Authors

Maria Davoren, Technological University DublinFollow
Eva Herzog, Technological University DublinFollow
Alan Casey, Technological University DublinFollow
Benjamin Cottineau, Technological University Dublin
Gordon Chambers, Technological University DublinFollow
Hugh Byrne, Technological University DublinFollow
Fiona Lyng, Technological University DublinFollow

Document Type

Article

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Publication Details

Published in Toxicology in Vitro 21 (2007) 438-448. Available online at www.sciencedirect.com

Abstract

This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lyso-somal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie bril-liant (CB) blue assay. Supernatants were also assayed for Adenylate Kinase (AK) release and Interleukin 8 (IL-8) which indicated a loss of cell membrane integrity and an in Xammation response respectively.

DOI

https://doi.org/10.1016/j.tiv.2006.10.007

Recommended Citation

Davboren, Maria et al: In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells. Toxicology in Vitrovol. 21, no. 3, pp. 438-448. doi:10.1016/j.tiv.2006.10.007