Document Type

Article

Disciplines

2.2 ELECTRICAL, ELECTRONIC, INFORMATION ENGINEERING

Publication Details

https://ieeexplore.ieee.org/abstract/document/10123402/keywords#keywords

R. -L. Xia et al., "Rapid Detection of SARS-CoV-2 Nucleocapsid Protein by a Label-Free Biosensor Based on Optical Fiber Cylindrical Micro-Resonator," in IEEE Sensors Journal, vol. 23, no. 12, pp. 12511-12518, 15 June15, 2023,

doi: 10.1109/JSEN.2023.3274110

Abstract

The current global outbreak of coronavirus (COVID-19) continues to be a severe threat to human health. Rapid, low-cost, and accurate antigen detection methods are very important for disease diagnosis. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) nucleocapsid protein (N-Protein) is often used as the diagnostic and screening for coronavirus detection. To this end, we propose and experimentally validate a highly sensitive whispering gallery mode (WGM) optical cylindrical micro-resonator (CMR) for bio immunoassay detection. To study the biokinetic process of immunoassay, the surface of the WGM micro-resonator is functionalized with N-Protein monoclonal antibody (N-Protein-m Ab), which led to the specific detection of N-Proteins. The spectral characteristics of the WGM resonance dip were investigated, and it is found that the transmission spectrum of WGM shows a monotonically increasing red-shift as a function of recording time. The WGM red-shift is due to the antibody-antigen reaction and can be used for the analysis of the immunoassay process. The wavelength shift is shown to be proportional to the concentration of N-Protein, which ranges between 0.1 and 100 μg /mL. Finally, different types of samples (concentration of 10 μg /mL of N-Protein) were prepared and tested to simulate the specificity of the sensor in the practical application environment. This method has the merits of a rapid assay, lower expense, easy preparation, and miniaturization, which makes the sensor have the potential for broad applications in the field of biochemistry and biomedical detection.

DOI

https://doi.org/10.1109/JSEN.2023.3274110

Funder

National Natural Science Foundation of China; Natural Science Foundation of Jiangxi Province; Research and Development Projects of the Ministry of Science and Technology of China Number;Royal Society International Exchanges 2020 Cost Share (NSFNanchang Hangkong University Graduate Student Innovation Special Fund ProjeNational Natural Science Foundation of the China-Royal Society Exchange Program

Creative Commons License

Creative Commons Attribution-Share Alike 4.0 International License
This work is licensed under a Creative Commons Attribution-Share Alike 4.0 International License.

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