Document Type
Article
Rights
Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence
Disciplines
3.2 CLINICAL MEDICINE
Abstract
Raman spectroscopy has numerous applications in the field of biology. One such application is the simultaneously measurement of the concentration of multiple biochemical components in low volume aqueous mixtures, for example, a single drop of blood serum. Over twenty years ago, it was shown for the first time that it was possible to estimate the concentration of glucose, urea, and lactic acid in mixture by combining Raman Spectroscopy with Partial Least Squares Regression analysis. This was followed by numerous contributions in the literature designed to increase the number of components and reduce the limits of concentration that could be simultaneously measured using Raman spectroscopy, by developing various optical architectures to maximise the signal to noise ratio. The aim of this paper is to demonstrate the potential of a confocal Raman microscopy system for multicomponent analysis for the case of physiologically relevant mixtures of glucose, urea, and lactic acid.
DOI
https://doi.org/10.1117/12.2307550
Recommended Citation
Zhengyuan, T., BarTon, S., Ward, T., Lowry, J., Doran, M., Byrne, H.J. & Hennelly, B. (2018). Quantifying the concentration of glucose, urea, and lactic acid in mixture by confocal Raman microscopy. , 106850V (17 May 2018). doi:10.1117/12.2307550
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Publication Details
Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106850V (2018) https://doi.org/10.1117/12.2307550
Event: SPIE Photonics Europe, 2018, Strasbourg, France