ORCID
- Probal Basu: 0000-0001-5163-8002
Abstract
The study emphasizes an in-depth in vitro efficiency analysis of bacterial cellulose (BC)-polyvinylpyrrolidone (PVP) composite hydrogel scaffolds which are doped with different concentrations of β-tri-calcium phosphate (β-TCP) and hydroxyapatite (HA). The hydrogels are prepared with the natural polymer, BC, and synthetic polymer, PVP. Polyethylene glycol (PEG) is also used as an anti-cytotoxic agent and agar is used as a gelling agent. β-TCP and HA were given in the ratio (w/w): 20:80 (BC-PVP- β-TCP/HA_20:80), 40:60 (BC-PVP-β-TCP/HA_40:60), 50:50 (BC-PVP-β-TCP/HA_50:50). In vitro efficacy of the different tri-calcium phosphate (β-TCP) and hydroxyapatite BC-PVP composite hydrogel scaffolds are analyzed through comparative analysis of the cell viability with human osteosarcoma cell line, Saos-2 and human adipose derived mesenchymal stem cells (hMSC). Notable cell viability is found with the scaffolds. Additionally, Ki-67 protein expression confirmed the cell proliferation without any significant cyto-pathological effects. Furthermore, significant expression of alkaline phosphatase (ALP) was observed with BC-PVP- β-TCP/HA_20:80 and BC-PVP- β-TCP/HA_50:50 scaffolds. Finally, SEM study indicated the notable adhesion and proliferation of Saos-2 on to the surface of the hydrogel scaffolds. Thus, these studies indicate the in vitro effectiveness of different tri-calcium phosphate (β-TCP) and hydroxyapatite doped BC-PVP composite hydrogel scaffolds and therefore recommended for further studies for bone tissue repair application.
Keywords
Bacterial cellulose, Biocompatibility, Bone regeneration, Hydrogel, In vitro characterization
DOI Link
Publication Date
2026-01-01
Publication Title
Emergent Materials
Volume
9
Issue
1-3
ISSN
2522-5731
Deposit Date
2026-05-11
Funding
Open access publishing supported by the institutions participating in the CzechELib Transformative Agreement.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Additional Links
Recommended Citation
Basu, Probal; Saha, Nabanita; Alexandrova, Radostina; Lilova, Boika Andonova; Argirova, Maya; and Saha, Petr, "In vitro efficiency analysis of different β-tri-calcium phosphate and hydroxyapatite doped bacterial cellulose-polyvinylpyrrolidone composite hydrogel for bone tissue repair" (2026). Research Outputs: 2025-Present. 17.
https://arrow.tudublin.ie/engschmecro/17