Author ORCID Identifier
Document Type
Article
Disciplines
Microbiology, Nano-materials, Nano-processes
Abstract
Antimicrobial resistance (AMR), caused by microbial infections, has become a major contributor to morbid rates of mortality worldwide and a serious threat to public health. The exponential increase in resistant pathogen strains including Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) poses significant hurdles in the health sector due to their greater resistance to traditional treatments and medicines. Efforts to tackle infectious diseases caused by resistant microbes have prompted the development of novel antibacterial agents. Herein, we present selenium and copper oxide monometallic nanoparticles (Se-MMNPs and CuO-MMNPs), characterized using various techniques and evaluated for their antibacterial potential via disc diffusion, determination of minimum inhibitory concentration (MIC), antibiofilm, and killing kinetic action. Dynamic light scattering (DLS), scanning electron microscopy (SEM/EDX), and X-ray diffraction (XRD) techniques confirmed the size-distribution, spherical-shape, stability, elemental composition, and structural aspects of the synthesized nanoparticles. The MIC values of Se-MMNPs and CuO-MMNPs against S. aureus and E. coli were determined to be 125 µg/mL and 100 µg/mL, respectively. Time–kill kinetics studies revealed that CuO-MMNPs efficiently mitigate the growth of S. aureus and E. coli within 3 and 3.5 h while Se-MMNPs took 4 and 5 h, respectively. Moreover, CuO-MMNPs demonstrated better inhibition compared to Se-MMNPs. Overall, the proposed materials exhibited promising antibacterial activity against S. aureus and E. coli pathogens.
DOI
doi.org/10.3390/biomedicines12050994
Recommended Citation
Rasheed, Rida; Bhat, Abhijnan School of Food Science and Environmental Health; Singh, Baljit MiCRA Biodiagnostics Technology Gateway and Health, Engineering & Materials Sciences (HEMS) Hub; and Tian, Furong Nanolab Research Centre, FOCAS Research Institute, Technological University Dublin, "Biogenic Synthesis of Selenium and Copper Oxide Nanoparticles and Inhibitory Effect against Multi-Drug Resistant Biofilm-Forming Bacterial Pathogens" (2024). Articles. 26.
https://arrow.tudublin.ie/diraaart/26
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Publication Details
https://www.mdpi.com/2227-9059/12/5/994#:~:text=The%20suppression%20of%20biofilm%20and,analyzed%20using%20a%20cell%20imager.
https://doi.org/10.3390/biomedicines12050994