Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma

Authors

Cássio A. Lima, Instituto de Pesquisas Energéticas e Nucleares
Luciana Correa, University of São Paulo
Hugh Byrne, Technological University DublinFollow
Denise M. Zezell, Instituto de Pesquisas Energéticas e Nucleares

Document Type

Conference Paper

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

1.3 PHYSICAL SCIENCES, Optics

Publication Details

Proc. SPIE 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII, 1086812 (26 February 2019)

Abstract

In this study, FTIR spectroscopy was used to evaluate the overall biochemical status of necrotic tissue areas of cutaneous squamous cell carcinoma chemically-induced on mice. FTIR hyperspectral image collected from specimen showed high correlation with the photomicrograph obtained by light microscopy, in which we were able to identify clusters associated to keratin, necrosis and regions with no tissue. Alterations in the protein content were documented in the necrotic tissue areas, indicating changes on protein conformation.

DOI

https://doi.org/10.1117/12.2510711

Recommended Citation

Cássio A. Lima, Luciana Correa, Hugh J. Byrne, Denise M. Zezell, "Label-free infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma," Proc. SPIE 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII, 1086812 (26 February 2019); DOI: 10.1117/12.2510711