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2.10 NANO-TECHNOLOGY, Toxicology, Public and environmental health
There is evidence that nanoparticles (NP) cross epithelial and endothelial body barriers. We hypothesized that gold (Au) NP, once in the blood circulation of pregnant rats, will cross the placental barrier during pregnancy size-dependently and accumulate in the fetal organism by 1. transcellular transport across the hemochorial placenta, 2. transcellular transport across amniotic membranes 3. transport through ~20 nm wide transtrophoblastic channels in a size dependent manner. The three AuNP sizes used to test this hypothesis are either well below, or of similar size or well above the diameters of the transtrophoblastic channels.
We intravenously injected monodisperse, negatively charged, radio-labelled 1.4 nm, 18 nm and 80 nm 198AuNP into pregnant rats on day 18 of gestation and in non-pregnant control rats and studied the biodistribution in a quantitative manner based on the radio-analysis of the stably labelled 198AuNP after 24 hours.
We observed significant biokinetic differences between pregnant and non-pregnant rats. AuNP fractions in the uterus of pregnant rats were at least one order of magnitude higher for each particle size roughly proportional to the enlarged size and weight of the pregnant uterus. All three sizes of 198AuNP were found in the placentas and amniotic fluids with 1.4 nm AuNP fractions being two orders of magnitude higher than those of the larger AuNP on a mass base. In the fetuses, only 1.4 nm and 18 nm AuNP were detected, but no 80 nm AuNP. These data show that no AuNP entered the fetuses from amniotic fluids within 24 hours but indicate that AuNP translocation occurs across the placental tissues either through transtrophoblastic channels and/or via transcellular processes.
Our data suggest that the translocation of AuNP from maternal blood into the fetus is NP-size dependent which is due to mechanisms involving (1) transport through transtrophoblastic channels – also present in the human placenta – and/or (2) endocytotic and diffusive processes across the placental barrier.
Semmler-Behnke et al. (2014) Size dependent translocation and fetal accumulation of gold nanoparticles from maternal blood in the rat, Particle and Fibre Toxicology, vol. 11, no.33 doi:10.1186/s12989-014-0033-9