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1.6 BIOLOGICAL SCIENCES
Pandoraeasp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damageof the lungs. The molecular mechanisms underlying the high pathogenicity ofPandoraeaspecies are still largely unknown. AsGram-negatives,Pandoraeasp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers aninflammatory response aimed at the bacterial eradication from the infected tissues. The degree of the inflammatory responsestrongly relies on the fine structure of the LPS and, in particular, of its glycolipid moiety, i.e. the lipid A. Here we report thestructure of the lipid A isolated from the LPS of a chronic strain ofP. pulmonicola(RL 8228), one of the most virulent identifiedso far among thePandoraeaspecies. Our data demonstrated that the examined chronic strain produces a smooth-type LPS with acomplex mixture of hypoacylated lipid A species displaying, among other uncommon characteristics, the 2-hydroxylation ofsome of the acyl chains and the substitution by an additional glucosamine on one or both the phosphate groups.
Di Lorenzo, F. et al, (2020) A chronic strain of the cystic fibrosis pathogen Pandoraeapulmonicola expresses a heterogenous hypo-acylated lipid A, Glycoconjugate Journal, DOI: 10.1007/s10719-020-09954-8