Document Type
Article
Rights
This item is available under a Creative Commons License for non-commercial use only
Disciplines
1.4 CHEMICAL SCIENCES
Abstract
A study of a dinitro-diester calix[4]arene (5,17-(3-nitrobenzylideneamino)-11,23-di-tert-butyl- 25,27-diethoxycarbonyl methyleneoxy-26,28-dihydroxycalix[4]arene) interaction with calf-thymus DNA was carried out using several techniques. The measurements were done at various molar ratios X=[calixarene]/[DNA]. Results show diverse changes in the DNA conformation depending on the X value. Thus, at low macrocycle concentration, the calixarene binds to the polynucleotide. This interaction, mainly in groove mode, weakens the hydrogen bonds between the base pairs of the helix inducing the denaturation of the double strands, as well as the condensation of the macromolecule, from an extended coil state to a globular state. An opposite effect is observed at X molar ratios higher than 0.07. The de-condensation of DNA happens, that is, the transition from a compact state to a more extended conformation, probably due to the stacking of calixarene molecules in the solution. Results also show the importance of making a proper choice of the system under consideration.
Recommended Citation
Deasy, M., Lopez-Cornejo. & Ostos, F.J. (2015). Binding of DNA by a dinitro-diester calix[4]arene: denaturation and condensation of DNA. Colloids Surf B Biointerfaces, vol. 127, pg. 65-72. doi:10.1016/j.colsurfb.2015.01.019.
DOI
https://doi.org/10.1016/j.colsurfb.2015.01.019
Publication Details
Colloids and Surfaces B: Biointerfaces 2015, 127, 65-72.