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Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence


1.6 BIOLOGICAL SCIENCES, Cell biology,, Biochemistry and molecular biology, Biomaterials, 3.2 CLINICAL MEDICINE, Oncology, 3.3 HEALTH SCIENCES, Public and environmental health, 3.4 HEALTH BIOTECHNOLOGY, Technologies involving the manipulation of cells,, tissues, organs or the whole organism, Biomaterials

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Plasma Processes and Polymers

Open access


The aim of this study was to determine the effects of a pin‐to‐plate cold atmospheric plasma (CAP) on U‐251 MG three‐dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species(ROS) plays a major role, while H2O2plays a partial role in CAP‐induced cytotoxicity in tumour-spheres. We conclude that ROS‐dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.



Science Foundation Ireland (SFI)