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Purpose: The objective of this study is to investigate whether cell culture medium is 40 a biologically relevant exposure medium that can be employed in non-ionising photobiological investigations. 42 Methods: The effect of solar simulated irradiation on cell culture medium and its ability to elicit cell death was studied. The role of reactive oxygen species (ROS), cell 44 secreted factors, and the contribution of individual components of the medium were investigated. 46 Results: Cell death was found to be primarily mediated through the formation of ROS via riboflavin photosensitisation and degradation in the cell culture medium. Phenol 48 red was found to significantly reduce the cell killing ability of riboflavin. Exposures in riboflavin free medium resulted in significantly increased cell survival compared to 50 identical exposures in riboflavin containing medium. Conclusions: This study has shown that solar radiation toxicity is augmented by cell 52 culture medium due to the presence of riboflavin. Results suggest that exposures performed in phenol red free medium may serve to increase phototoxic effects if 54 riboflavin is present. Riboflavin free media is recommended for solar radiation investigations to eliminate concerns regarding riboflavin photosensitisation and nutrient 56 deprivation.
Maguire, A. et al. (2011)Medium mediated effects increase cell killing in a human keratinocyte cell line exposed to solar simulated radiation, Int J Radiat Biol. 2011 Jan;87(1):98-111. doi: 10.3109/09553002.2010.518210.
Department of Education & Science