Document Type

Book Chapter


This item is available under a Creative Commons License for non-commercial use only


1. NATURAL SCIENCES, Microbiology, Biochemistry and molecular biology

Publication Details

Volume 1512 of the series Methods in Molecular Biology pp 107-115


Studies of the interaction of bacteria with mucus secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high throughput manner.