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1. NATURAL SCIENCES, Microbiology, Biochemistry and molecular biology
Studies of the interaction of bacteria with mucus secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high throughput manner.
Naughton, J., Clyne, M., Duggan, G. & Burke, B. (2017). Methods to assess the direct interaction of C. jejuni with mucins. In Methods in Molecular Biology,vol. 1512, pp. 107-115. doi:10.1007/978-1-4939-6536-6_10
Science Foundation Ireland