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Virology, Biochemistry and molecular biology
Equine inﬂuenza is a cause of epizootic respiratory disease of the equine. The detection of equine inﬂuenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two inﬂuenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine inﬂuenza virus, 50% egg infectious dose and 50% tissue culture infectious dose. There was a signiﬁcant positive correlation (P < 0.05) between the quantitative RT-PCR and both of these assays.
Quinlivan, M., Dempsey, E.,Ryan , F., Arkins,S., Cullinnane, A. : Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus. JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 2005, p. 5055–5057 Vol. 43, No. 10 0095-1137/05 doi:10.1128/JCM.43.10.5055–5057.2005