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Biochemistry and molecular biology

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Dheeman DS, Frias JM, Henehan GTM (2010) Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304. Journal of Industrial Microbiology & Biotechnology 37 (1) 1-17.



Among several lipase producing actinomycete strains screened, Amycolatopsis mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. Culture condition and nutrient source modification studies involving carbon sources, nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast extract (5:1) as nitrogen sources. In optimal culture conditions the lipase activity was enhanced 12-fold with a 2-fold increase in lipase specific activity. The lipase showed maximum activity at 60 °C and pH 8.0. The enzyme was stable between pH 5.0–9.0 and temperatures up to 60 °C. Lipase activity was significantly enhanced by Fe3+ and strongly inhibited by Hg2+. Li+, Mg 2+ and PMSF significantly reduced lipase activity, whereas other metal ions had no significant effect at 0.01 M concentration. A. mediterranei DSM 43304 lipase exhibited remarkable stability in the presence of a wide range of organic solvents at 25% (v/v) concentration for 24h. These features render this novel lipase attractive for potential biotechnological applications in organic synthesis reactions.



ABBEST Research Scholarship,Technological University Dublin (PB 03557/2007).

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