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1.4 CHEMICAL SCIENCES
The susceptibility of Candida albicans biofilms to a non-thermal plasma treatment has been investigated in terms of growth, survival and cell viability by a series of in vitro experiments. For different time periods , the C. albicans strain SC5314 was treated with a microwave-induced plasma torch (MiniMIP). The MiniMIP treatment had a strong effect (reduction factor (RF) = 2.97 after 50 s treatment) at a distance of 3 cm between the nozzle and the superior regions of the biofilms. In addition, a viability reduction of 77% after a 20 s plasma treatment and a metabolism reduction of 90% after a 40 s plasma treatment time were observed for C. albicans. After such a treatment , the biofilms revealed an altered morphology of their cells by atomic force microscopy (AFM). Additionally , fluorescence microscopy and confocal laser scanning microscopy (CLSM) analyses of plasma-treated biofilms showed that an inactivation of cells mainly appeared on the bottom side of the biofilms. Thus, the plasma inactivation of the overgrown surface reveals a new possibility to combat biofilms.
Handorf, O. et al (2019) Antimicrobial Effects of Microwave-Induced Plasma Torch (MiniMIP) Treatment on Candida Albicans Biofilms, Microbial Biotechnology 12(69) · July 2019. DOI:10.1111/1751-7915.13459