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In this study, brewer’s spent grain (BSG) was subjected to a range pretreatments to study the effect on reducing sugar yield. Glucose and xylose were found to be the predominant sugars in BSG. Brewers spent grain was high in cellulose (19.21g/100g of BSG) and lignin content (30.84g/100g of BSG). Microwave assisted alkali (MAA) pretreatment was found to be the most effective pretreatment for BSG, where the pretreatment was conducted at 400W for 60s. A maximum reducing yield was observed with high biomass loading (1g/10ml), cellulase (158.76 μl/10ml), hemicellulase (153.3 μl/10ml), pH (5.4) and an incubation time (120h). Upon enzymatic hydrolysis, MAA pretreated BSG yielded 228.25 mg of reducing sugar/g of BSG which was 2.86-fold higher compared to native BSG (79.67 mg/g of BSG); simultaneously BSG was de-lignified significantly. The changes in functional groups, crystallinity and thermal behaviour was studies by means of FTIR, XRD and DSC, respectively.
Ravindran, R., Jaiswal, S., Abu-ghannam, N. & Jaiswal, A. K. (2017). A comparative analysis of pretreatment strategies on the properties and hydrolysis of Brewers' spent grain. Bioresource Technology, June 2017 doi:10.1016/j.biortech.2017.06.039
Technological University Dublin (DIT), Dublin, Ireland