Quantitative Assessment of Blood Coagulation by Cold Atmospheric Plasma

Caitlin Heslin, Dublin Institute of Technology
Daniela Boehm, Dublin Institute of Technology
Vladimir Milosavljevic, Dublin Institute of Technology
Michelle Laycock, Dublin Institute of Technology
Patrick Cullen, Dublin Institute of Technology
Paula Bourke, Dublin Institute of Technology

Document Type Article

Plasma Medicine 4(1-4): 153-163 (2014)

Because of the publisher's policy we are unable to offer any version of this article. However, please note that this article will be available on PLOS 12 months after publication.


Cold atmospheric plasma (CAP) is produced by ionizing a chosen gas, thereby creating charged and reactive species. The reactive species generated are capable of inducing a range of biomedically relevant interactions including blood coagulation. However, the underlying biochemical processes of plasma-assisted blood coagulation are largely unknown, and data quantifying blood clot formation or the impact of system parameters on the intensity of the blood clot are scarce. In this study, blood coagulation was quantified by measuring hemoglobin absorbance. System parameters of the kINPen plasma jet were investigated and compared, including treatment time, distance from the plasma source and gas flow rate. These investigations were combined with optical emission spectroscopy to associate the species generated in the plasma effluent with the effect on coagulation efficiency. In this study, we have proposed a method to quantitatively assess plasma-induced blood coagulation to directly compare the clotting capabilities of other plasma systems and to explicate the mechanism of plasma-assisted blood coagulation.