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3. MEDICAL AND HEALTH SCIENCES
Modern models of radiobiological effects include mechanisms of damage initiation, sensing and repair, for those cells that directly absorb ionizing radiation as well as those that experience molecular signals from directly irradiated cells. In the former case, the effects are termed targeted effects while, in the latter, non-targeted effects. It has emerged that phenomena occur at low doses below 1Gy in directly irradiated cells which are associated with cell-cycle dependent mechanisms of DNA damage sensing and repair. Likewise in non-targeted bystander irradiated cells the effect saturates at 0.5Gy. Both effects at these doses challenge the limits of detection of vibrational spectroscopy. In this paper, a study of the sensing of both targeted and non-targeted effects in HaCaT human keratinocytes irradiated with gamma-ray photons is conducted with vibrational spectroscopy.
Meade, A., Howe, O. & Unterreiner, V. (2015). Vibrational spectroscopy in sensing radiobiological effects: analyses of targeted and non-targeted effects in human keratinocytes. Faraday Discussions, vol. 187, pp. 213.34. 10.1039/c5fd00208g.