Title
Reflection Contributions to the Dispersion Artefact in FTIR Spectra of Single Biological Cells
Document Type
Article
Rights
Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence
Abstract
Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.
Recommended Citation
Bassan, P. et al (2009) Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells. Analyst, vol.134, pp.1171-1175. DOI: 10.1039/b821349f
DOI
https://doi.org/10.1039/b821349f
Publication Details
Published in Analyst, 2009, 134, 1171-1175. DOI: 10.1039/b821349f http://www.rsc.org/Publishing/Journals/AN/article.asp?doi=b821349f